PGLO

By adding a glowing gene to E-coli bacteria, we can make glow under UV light bacteria strains. 

Our hypothesis: 

1.  We prepare two different solutions, one with pGlo genes and one without. we add a culture of e-coli bacteria to each sample, then flash heat and freeze to encourage the polo dna to enter the e-coli cell membrane. because e coli is a eukaryotic animal, there are no cell nuclei to enter, it's relatively simple to get the foreign pGLO into the cell where it will be integrated during protein synthesis. 

2. we have four petri dishes

+/amp/ara, +/amp, -/amp, -/nothing else.

the sample bacteria cultures with pGLO added are placed in the + dishes. 

3. after incubating, we find that the dish containing arabinose grew bacteria that glowed, and the dish with ampicillin containing non-glowed/ ampicillin R bacteria didn't grow. the other two dishes grew to varying degrees. 

From the results we can see that the pGLO gene is activated by the presence of arabinose, because the only pGLO petri dish that glowed contained arabinose.